Atlas of plant and animal histology

Choose true or false. Each right answer scores 1, and each wrong answer scores -1. Not answered questions score 0.

1) The study of tissues with optical microscopes needs the samples to be sectioned.

2) The devices to section the tissues are called microtomes.

3) The rotary microtome is commonly used to obtain sections from paraffin-embedded samples.

4) To section paraffin-embedded samples, the paraffin block has to be extended by heating.

5) The trimming of the paraffin blocks is intended to obtain thinner sections.

6) Sections are mounted on slides covered with adherent chemical coats.

7) The temperature to extend the paraffin sections is about 65°C.

8) Vibratome is intended for non-embedded tissue samples.

9) Floating sections means that they are processed non-attached to slides.

10) The samples sectioned with any microtome have to be chemically fixed.

11) Cryoprotection is a critical step to prevent tissue damage when using freezing microtomes.

12) Sections obtained with the freezing microtome can be thinner than those obtained with the cryostat.

13) Semithin sections are thinner than 100 nanometers.

14) Semithin and ultrathin sections are obtained with the ultramicrotome.

15) The more hardened the sample, the thinner the section to be obtained.

16) The same type of blades can be used for both ultramicrotomes and cryostats.

17) Sections obtained with the ultramicrotome can be processed attached to slides.