Alcian blue is a basic dye used to stain carbohydrates in animal tissues and primary cell walls in plant tissues. The blue color is provided by the copper present in the dye molecule.
Procedure
1 g Alcian blue (C.I. 74240)
3 ml glacial acetic acid
97 ml distilled water
Notes
Mix thoroughly the components of the solution. I can be stored for years. An optimal staining is achieved at about pH 2. Thus, adjust the pH with acetic acid before use.
Products
Alcian blue (C.I. 74240)
Glacial acetic acid
Distilled water
Labware
Test tube
Balance
Magnetic stirrer
Flasks
Bottles