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The cell. 7. Cytosol. Cytoskeleton.

ACTIN FILAMENTS

Actin filaments are one type of the cytoskeleton components. In animal cells, actin filaments use to be located close to the plasma membrane (Figures 1 and 2), but their distribution and organization depend much on the cellular type. Actin filaments perform many functions in the cell. Cells need actin filament for dividing, endocytosis, phagocytosis, organelles communication. In animal cells, actin filament provides mechanical support for maintaining or changing the cellular shape.

 Actin
Figure 1. Actin filaments (in green ) in cells in culture. Notice the higher amount of filaments at the cell periphery. Nuclei are in blue. (Pictures by Sheila Castro Sánchez. Dept. Biochemistry, Genetics and Imnmunology. University of Vigo).
 Actin
Figure 2. Transmission electron microscopy image showing actin filament bundles close to the plasma membrane.

1. Structure

 Actin filament
Figure 3. An actin filament is depicted showing the helical arrangement of actin proteins. Microfilaments are polarized structures with two ends (minus and plus ends) showing different polymerization and depolymerization rates. Free ATP-actin proteins are prone to join to the microfilament by polymerization. After some time in the filament, the ATP is hydrolyzed producing ADP-actin proteins. The ADP-actin form constitutes the majority of the filament. (Adapted from Pollard and Earnshaw, 2007).

Actin filaments are built by polymerization of globular actin proteins (Figure 3), which can be found in two isoforms: alpha- and beta-actin. Alpha-actin is abundant in muscle cells. Beta-actin is the most frequent isoform and is found in most animal cells. Actin is a very abundant protein in the cytosol, about 10 % of the total cytosolic protein content. In total cytosolic actin protein pool, some are found as part of the actin filaments (known as F-actin), and the remaining is free in the cytosol (known as G-actin).

Actin filaments are 7 nm in thickness. This is lower than the thickness of the other cytoskeleton filaments, microtubules and intermediate filaments. That is why actin filaments are also known as microfilaments. Every actin filament has a minus end and a plus end, which means that they are polarized filaments. At the plus end, the polymerization, addition of new actin proteins, is more frequent than depolymerization, whereas at the minus end depolymerization is more frequent. The increase and decrease in the microfilament length is by polymerization and depolymerization, respectively. In the cell, these changes are happening all the time, as well as nucleation of new microfilaments, but complete depolymerizations as well. Actin filaments are the most dynamic component of the cytoskeleton.

The cytosol environment and the concentration of free actin proteins in the cytosol prevent the spontaneous assembling of actin filaments. Hence, the formation of new microfilaments relies on nucleation protein complexes such as Arp2/3 and formins. Arp2/3 proteins work as nucleation sites for new microfilaments, whereas formins stabilize transient spontaneous associations of actin proteins, boosting the formation and elongation rate of microfilaments. This control of microfilament formation is very useful for the cell because new microfilaments are formed when and where they are needed by precisely placing nucleation protein complexes.

2. Organization

A salient feature of actin filaments is that they are highly adaptable: are formed and removed easily, and are associated between each other in many ways to form 3D scaffolds. This versatility relies on more than 100 different modulator protein types or actin associated proteins (Figure 4). These proteins regulate filament polymerization and depolymerization rates, nucleation of new filaments, destruction of existing filaments, as well as 3D organization. Actually, there is no naked actin filaments or free actin proteins in the cytosol, but they are always linked to some associated protein.

 Actin organization
Figure 4. Polymerization and depolymerization of actin filaments are under the control of many actin associated proteins. In this image, several organizations of actin filaments are depicted, as well as the actin associated proteins that influence these arrangements. (Modified from Pollar and Earnshaw, 2007).

Proteins associated to actin filaments can be divided according to their functions. a) Modulating polymerization. Some proteins, such as profilin, join free actin (G-actin) and boost actin filament polymerization. Other proteins, such as thymosin, join free actin proteins and hamper the polymerization process by preventing the spontaneous polymerization of actin filaments. b) Modulating the spatial organization. There are proteins, such as fimbrin and alpha-actinin, making cross-bridges between actin filaments to form bundles of actin filaments, whereas other proteins like filamin make possible the arrangement of actin filaments in reticular structures. c) Some actin binding proteins, such as cofilin, katanin, and gelsolin, break and reorganize the actin filaments. d) There are also proteins that are intermediaries between actin filaments and other proteins. For example, tropomyosin mediates interaction between actin filament and myosin in the muscle cells. e) Some proteins are in charge of anchoring actin filaments to other cellular structures like membranes and other components of the cytoskeleton. Some actin associated proteins may perform more than one function.

There are additional elements modulating the effect of actin associated proteins on actin filaments behavior, such as calcium concentration, activity of Rho-GTPases, presence of lipids, and higher or lower gen expression of their genes. There are also drugs that influence the polymerization of actin filaments. For example, cytochalasin inhibit polymerization, whereas phalloidin inhibit depolymerization.

3. Myosin

Many functions performed by actin filaments relies on a type of motor proteins known as myosins. These proteins can move cargoes along the actin filament toward the plus end. The energy comes from ATP hydrolysis. Besides dragging cargoes, myosins can also slide two actin filaments one over the other in opposite directions. Furthermore, if the myosin is anchored, then the motor traction can move the actin filament. Myosins are actually a diverse family of proteins, with more than 40 members in mammals.

4. Functions

Cell shape

Underneath the plasma membrane, there is a cortical layer of actin filaments of about 100 nm thick (Figure 5). They are interlocked between each other by associated proteins, and anchored to proteins and lipids of the plasma membrane. There is also myosins generating mechanical forces that push actin filaments and change the position of plasma membrane. This actin filaments layer makes cell both withstand and generate mechanical forces, influencing, in this way, the cell shape. Animal cells lack cell wall, so that cell shape largely depends on the actin filaments of the cortical cytoplasm.

 Actin filaments organization
Figure 5. Actin filaments organized in a layer beneath the plasma membrane in animal cells.

The cell shape also depends on the adhesion contacts with the extracellular matrix and other adjoining cells (Figure 6). Integrins mediate the adhesion of the cell to the extracellular matrix. In their cytosolic domain, integrins are connected to actin filaments, so there is a mechanical continuity between extracellular matrix and cytoskeleton. Tight junctions and adherent junctions are cell-cell junctions made up of adhesion transmembrane proteins, occludin/claudin and cadherin, respectively, that are also connected to actin filaments by intermediary proteins.

 Actin filaments organization
Figure 6. Some adhesion molecules are anchored to actin filaments by intermediary proteins.

Cell movement

Cells do not swim, but they crawl or trail across the tissues, as do, for example, the embryonic cells during development, amoeba when moving around, lymphocytes when heading toward damage tissues, and the growth cone of neurons when searching for their targets. For cell movement, several steps are needed: production and extension of cytoplasmic protrusions, adhesion of these protrusions to some elements of the environment, and drag the rest of the cell toward these anchoring points. The cellular protrusions are known as podia, and tere are lamellipodia when they are sheet-like, filopodia when they are thin and long, and lobopodia when they are thick and tubular. Actin filaments have a prominent role in the formation of these protrusions (Figure 7). Actually, it is actin polymerization what pushes plasma membrane outward and forms protrusions.

 Podia
Figure 7. Cellular extensions mediated by actin filaments and associated proteins.

Lamellipodia are rather flattened cell expansions originated by actin filament polimerization that, instead of bundles, form a branched scaffold. These expansions appear when cells are moving, during phagocytosis and macropinocytosis. Filopodia may emerge from lamellipodia or from other cell regions. Typical filopodia are a few µm in diameter and no more than 10 µm in length. They contain a few dozens of actin filaments organized as a bundle. Podosomes are cell expansions attached to the extracellular matrix through integrins, but they are also able to release metalloproteinases for extracellular matrix degradation. They contain a central branched scaffold of actin filaments surrounded by non-branched actin filaments. Podosomes may be mechanosensors that explore the environment and may also contribute to cell movement.

When these cell expansions contact any extracellular structure, either extracellular matrix macromolecules or other cell, they get anchored by adhesion proteins like cadherins. Once anchored, cell moves all the intracellular compartments and structures toward the adhesion points. The dragging force relies on the stress fibers, intracellular structures composed of actin filaments and myosin proteins (Figure 8).

 Stress fibers
Figure 8. Actin filament bundles organizes as stress fibers during cell movement.

Internal organization

Actin filaments are mostly found near the plasma membrane, in the so-called cortical region. In this position, they are involved in vesicle formation, macropinocytosis and phagocytosis (Figure 9).

Vesicles
Vesicles
 Actin filaments
Figura 9. Actin filaments are involved in vesicle formation, macropinocytosis and phagocytosis. Arrows indicate the direction of the outgrow.

Organelles are moved through the cytoplasm. Actin filaments, together with myosin motor proteins, help with the organelle movements (Figure 10). This role is very important in plant cells where most of the internal movements of organelles are carried out by actin filaments. The chloroplasts movement, process known as cyclosis, can be observed under the light microscope.

 Organelles movement
Figure 10. Actin filaments are used as "railways" by myosin when dragging and moving organelles .

Muscular contraction

In muscle cells, myosin II are associated between each other to form the thick myosin filaments, which show a polarity as if they were an arrow with two arrowheads, each in one end (Figure 11). In the striated muscle, each of these heads drag actin filaments toward the middle point of the myosin filament. This the molecular base for muscle cell contraction.

 Muscle contraction
Figure 11. Actin and myosin II filaments form the muscle cell sarcomere.

Cytokinesis

During cell division, the final strangling of the cytoplasm is produced by a ring of actin filaments that, helped by myosin II motor proteins, gets progressively smaller in diameter until the complete separation of the cytoplasm of the two new cells (Figure 12).

Cytokinesis
Cytokinesis.
 Cytokinesis
Figure 12. Actin filaments and myosin II strangle the cytoplasm during cytokinesis.

Microvilli

Microvilli are filiform expansions of the apical part of some cells that enormously increase the surface of the plasma membrane. For example, they can be found in the epithelium of the gut and in the proximal tube of the nephron. Each of these little expansions or microvillus are 1 to 2 µm height and around 0.1 µm thick. Each of them contains several dozens of actin filaments oriented parallel to the longitudinal axis of the microvillus (Figure 13). At the base of microvilli, in the peripheral cytoplasm, there is a molecular scaffold known as terminal web, which is composed of actin filaments that stabilizes the whole set of microvilli.

Microvilli
Microvilli
 Microvilli
Figure 13. Actin filaments are the skeleton of microvilli.
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