van Gieson picrofuchsin is part of the van Gieson trichrome staining. It stains fibers of the connective tissue, such as collagen and reticular fibers.
Procedure
Solution A
1 g acid picrofuchsin (C.I. 75290)
100 ml distilled water
Solution B
Saturated solution of picric acid
Notes
A and B solutions are mixed in 1:45 proportion. This solution can be immediately used or left for maturation during several weeks. In any case, 0.25 ml of acid chloride is sometimes added before use.
Products
Acid fuchsin (C.I. 75290)
Acid chloride
Picric acid saturated solution
Distilled water
Labware
Test tube
Balance
Magnetic stirrer
Flasks
Bottles