Light green (C.I. 42095) and fast green (C.I. 42053) are two dyes that can be easily dissolved in water. They are used to staining animal connective tissue in trichrome staining protocols, and also combined with safranin for plant tissues. The staining properties of these dyes are similar, so they can be used indistinctly. However, the staining durability by fast green may last longer.
Procedimiento
Light green (Masson's trichrome)
2 g light green (C.I. 42095)
2 ml acetic acid
untill 98 ml with distilled water
Fast green (Masson's trichrome)
0,2 g fast green (C.I. 42053)
100 ml distilled water
Fast green (for plant tissues)
1 g fast green (C.I. 42053)
50 ml 100º ethanol
50 ml clove essence oil
Products
Distilled water
Light green (C.I. 42095)
Fast green (C.I. 42053)
Acetic acid
Clove essence oil
Labware
Test tube
Balance
Magnetic stirrer
Flasks
Bottles