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Home / Techniques / Protocols / Cresyl violet

Techniques. Protocols

CRESYL VIOLET

Cresyl violet is a basic synthetic dye extensively used to stain the nervous tissue, although it is also suitable for staining mastocytes and cartilage. Cresyl violet is different from crystal violet, which is for bacteria Gram staining. In the nervous tissue, cresyl violet can be combined with luxol fast blue for myelin staining.

Procedure (a)

0,1 g cresyl violet

100 ml distilled water

1 or 2 (about 0,25 ml) drops of acetic acid

Procedure (b)

0,6 ml acetic acid in 100 ml of distilled water (A)

1, 36 mg sodium acetate in 100 ml of distilled water (B)

Acetate buffer: mix solutions A:B (9:1)

1 g cresyl violet in 100 ml distilled water

Working solution ... Acetate buffer: 1 % cresyl violet (1:1)

Notes

In the procedure B, adjust the pH of solution B to 3,7 before mixing with cresyl violet.

Products

resyl violet

Acetic acetic

Sodium acetate

Distilled water

Labware

Test tube

Flasks

Bottles

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